LS 174T人結腸腺癌細胞
描述：LS 174T是LS 180 (ATCC CL 187)結腸腺癌細胞株的胰蛋白酶化變種。 它比親本更易傳代，象LS 180一樣生成大量的癌胚抗原(CEA)。 電鏡研究表明有豐富的微絲和細胞質粘液素液泡。 直腸抗原3陽性。 p53抗原表達陰性，但mRNA表達陽性。 與ATCC CL-187來源于同一個腫瘤。LS 174T細胞角蛋白染色陽性。 癌基因c-myc, N-myc, H-ras, N-ras, Myb, 和 fos的表達呈陽性。 癌基因k-ras和sis的表達未做檢測。
動物種別：人
性別：女
組織來源：直腸；結直腸腺癌
形態：上皮細胞
培養基和添加劑：DMEM培養基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，90%；優質胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。
供應限制：A。僅供研究之用。
REFERENCE：Tom BH , et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041 Jakstys MM , et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980 Chen TR , et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615 Gastl GA , et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757 Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 Tsao H , et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066 Shibata D , et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat. Genet. 6: 273-281, 1994. PubMed: 8012390 Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996.
中文名稱：人結腸腺癌細胞
